PDF) A spreadsheet that calculates primer concentration in the final PCR reaction
SOLVED: 2. Design a PCR experiment to amplify a sequence of interest: Use the following reagent concentrations for calculating your cocktail. Fill in the chart below. A. Fill in the chart below
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SOLVED: Once you have prepared your primers, you need to consider how to constitute Depending on the need, we your PCR reaction: volume usually do PCR in 10-50 ul volume: Here we
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SOLVED: Design a PCR experiment to amplify a sequence of interest: Use the following reagent concentrations for lculating your cocktail: Fill in the chart below. REAGENT Final Test Reaction Control Reaction Master
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